Name | Bis-Tris Buffer(1.25M,pH6.5-6.8) |
Synonyms | Bis-Tris Buffer(1.25M,pH6.5-6.8) 2,2-BIS(HYDROXYETHYL)-(IMINOTRIS)-(HYDROXYMETHYL)-METHANE 2-BIS[2-HYDROXYETHYL]AMINO-2-[HYDROXYMETHYL]1,3-PROPANEDIOL Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane~Bis-TRIS Bis Tris Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane Bis-Tris (2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol) 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-3-propanediol Bis Tris, Molecular Biology Grade Bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane, Molecular Biology Grade |
CAS | 6976-37-0 |
EINECS | 230-237-7 |
InChIKey | OWMVSZAMULFTJU-UHFFFAOYSA-N |
Molecular Formula | C8H19NO5 |
Molar Mass | 209.24 |
Density | 1.346±0.06 g/cm3(Predicted) |
Melting Point | 104°C(lit.) |
Boling Point | 466.2±40.0 °C(Predicted) |
Water Solubility | soluble |
Solubility | H2O: 1M at20°C, clear, colorless |
Vapor Presure | 0Pa at 25℃ |
Appearance | Solution |
Color | Yellow to orange |
Odor | Odorless |
Maximum wavelength(λmax) | ['λ: 280 nm Amax: 0.15'] |
BRN | 2205275 |
pKa | 6.5(at 25℃) |
PH | 9.0-10.1 (20-25℃, 1M in water) |
Storage Condition | Inert atmosphere,Room Temperature |
Sensitive | Air Sensitive |
Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R21/22 - Harmful in contact with skin and if swallowed. R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S22 - Do not breathe dust. |
WGK Germany | 3 |
FLUKA BRAND F CODES | 10-23 |
TSCA | Yes |
HS Code | 29221980 |
pH range of acid-base indicator discoloration | 5.8 - 7.2 |
LogP | -2.26 at 20℃ |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
Use | for biological buffer buffer for detection of creatine kinase; also used to effectively protect hemoglobin in freeze-drying; Catholyte, used in IEF/2-D gel electrophoresis. |